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胃癌细胞中COX-2通过NF-κB/Snail调控E-cadherin表达的机制
作者:陈兆峰  黄珊珊  刘敏  李玲玲  刘小军  李海龙  姬瑞  郭庆红  周永宁 
单位:1. 兰州大学第一医院消化内科  甘肃 兰州 730000 
2.
 甘肃省胃肠病重点实验室  甘肃 兰州 730000 
3.
 东营市东营区人民医院肿瘤内科  山东 东营 257100 
关键词:胃肿瘤 环氧化酶2 NF-κB 钙黏着糖蛋白类 逆转录聚合酶链反应 肿瘤侵润 肿瘤细胞 培养的 
DOI:R735.2
出版年,卷(期):页码:2014,29(2):118-122
摘要:
目的 探讨COX-2调控E-cadherin表达的相关信号通路及分子机制,揭示COX-2与胃癌侵袭转移的关系及作用机制。方法 采用不同浓度的选择性COX-2抑制剂塞来昔布干预人胃癌细胞株SGC-7901不同时间后,应用实时荧光定量RT-PCR法和Western blot法检测SGC-7901细胞中COX-2、NF-κB、Snail及E-cadherin mRNA和蛋白的表达情况。结果 随着塞来昔布作用剂量的增大和干预时间的延长,COX-2、NF-κB和Snail mRNA及蛋白的表达量均显着下降(P<0.05),呈剂量和时间依赖性降低;E-cadherin mRNA及蛋白的表达量上升(P<0.05),呈剂量和时间依赖性升高。采用Spearman相关分析,显示COX-2与NF-κB及COX-2与Snail蛋白表达呈正相关性(r=0.881,P<0.01;r=0.839,P<0.01);COX-2与E-cadherin蛋白表达呈负相关性(r=-0.814,P<0.01)。结论 COX-2可能通过NF-κB/Snail信号通路调控E-cadherin的表达,进而参与胃癌的浸润转移过程。
Objective To investigate the mechanisms related to the expression of E-cadherin regulated by COX-2 in human gastric cancer cells.Methods Human gastric cancer SGC-7901 cells were treated with eyclooxygenase-2 (COX-2) inhibitor, celecoxib, at different concentrations for different durations.Western blot and real time quantitative RT-PCR were used to detect mRNA and protein expression of COX-2,NF-κB,Snail and E-cadherin.Results The mRNA and protein expressions of COX-2,NF-κB and Snail in SGC-7901 cells declined and those of E-eadherin increased significantly after treated with celecoxib (P<0.05), in a dose- and time-dependent manner.The expression of COX-2 was positively correlated with NF-κB (r=0.881,P<0.01) and Snail (r=0.839,P<0.01) level, and was negatively correlated with E- cadherin level (r=-0.814,P<0.01).Conclusion COX-2 may regulate the expression of E-cadherin through NF-κB and Snail signaling pathway during gastric cancer progression.
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