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钙池操纵性钙内流在非小细胞肺癌中的作用
作者:杨海虹  徐小明  张晨婷  邓秋华 
单位:1. 广州医科大学附属第一医院胸部肿瘤科  广东 广州 510120 
2.
 广州呼吸疾病国家重点实验室  广州呼吸疾病研究所  广东 广州 510120 
关键词: 非小细胞肺/病理学 钙通道  RNA 信使 细胞增殖 肿瘤侵润 
DOI:R734.2;R730.23
出版年,卷(期):页码:2015,30(5):413-417
摘要:
目的 探讨钙池操纵性钙通道(store-operated calcium channel,SOCC)介导的钙池操纵性钙内流(storeoperated calcium entry,SOCE)在非小细胞肺癌中的作用。方法 应用实时定量PCR方法和Western blot方法检测非小细胞肺癌组织和A549肺腺癌细胞中SOCC的mRNA和蛋白表达。应用Fura-2Am荧光素方法检测A549细胞的SOCE;经过不同浓度SOCE抑制剂处理A549细胞后,应用WST-1比色法和Transwell方法检测A549细胞的增殖和侵袭能力改变。结果 在非小细胞肺癌组织和A549细胞中有STIM1、ORAI1~3的mRNA和STIM1、ORAI1的蛋白表达,分化差的肿瘤组织比分化好的高表达ORAI1 mRNA(P=0.028);A549细胞中存在SOCE,并且SOCE抑制剂SKF-96365或NiCl2可抑制A549细胞的增殖和侵袭。结论 非小细胞肺癌组织中表达STIM1和ORAI1~3。A549肺腺癌细胞存在SOCE,并且由SOCC介导的SOCE在肺癌细胞生长和转移过程中起着重要的作用。
Objective To investigate the effect of store-operated calcium entry (SOCE) induced by store-operated calcium channels (SOCCs) in non-small-cell lung cancer (NSCLC). Methods The mRNA and protein expressions of SOCCs in human NSCLC tissues and lung adenocarcinoma A549 cell365bet娱乐官网网址s were detected with real-time PCR and Western blot, respectively. SOCE in A549 cells was examined with Fura-2Am method, and the growth and migration of A549 cells were determined by WST-1 assay and transwell assay after treated by SOCE inhibitors. Results STIM1 and ORAI1 proteins and STIM1 and ORAI1~3 mRNA were expressed in NSCLC tissues and A549 cells. Higher expression of ORAI1 mRNA was found in less differentiated NSCLC tissues (P=0.028). The mediation of SOCE by SOCCs was found in A549 cells. SOCE inhibitors, SKF-96365 and NiCl2, reduced the growth and migration of A549 cells significantly. Conclusion SOCE is mediated by STIM1 and ORAI1, which may be involved in the migration and growth of lung adenocarcinoma cells.
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