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miR-18a对肝癌细胞侵袭及迁移能力的影响及其机制
作者:何兴鸿  贺敏 
单位:荆门市中医医院肿瘤科  湖北 荆门 448000 
关键词: 肝细胞/药物疗法 肿瘤浸润 细胞运动 微RNAs/药理学 肝肿瘤/药物疗法 转染 肿瘤细胞 培养的 
DOI:R735.7;R730.53
出版年,卷(期):页码:2017,32(3):208-211,212
摘要:

目的 探讨miR-18a对肝癌患者血清肝癌细胞侵袭及迁移的影响及其作用机制。方法 细胞经培养、传代、冻存、复苏后采用脂质体介导转染法进行转染。肝癌细胞株HepG2.2.15及HepG2分别转染miR-18a inhibitor和miR-18a inhibitor阴性对照24 h后,应用transwell侵袭和迁移实验测定肝癌细胞的侵袭和迁移能力。肝癌细胞株HepG2和HepG2.2.15分别转染miR-18a inhibitor、miR-18a inhibitor阴性对照、共转染miR-18ainhibitor和Dicer siRNA以及共转染miR-18a inhibitor和Dicer siRNA阴性对照48 h后,应用Western blot法比较各组Dicer1蛋白表达的差异,应用transwell侵袭和迁移实验比较各组细胞侵袭以及迁移能力的差异。结果 转染miR-18a inhibitor后,HepG2和HepG2.2.15细365bet娱乐官网网址胞的侵袭和迁移能力均降低,差异均具有统计学意义(均P<0.05)。转染miR-18a inhibitor组Dicer1蛋白表达水平较转染miR-18a inhibitor 阴性对照组升高,差异具有统计学意义(P<0.05)。共转染Dicer siRNA+miR-18a inhibitor组Dicer1蛋白表达水平较Dicer siRNA 阴性对照+miR-18a inhibitor组降低,差异具有统计学意义(P<0.05);细胞的侵袭和迁移能力均增强,差异均具有统计学意义(均P<0.05)。结论 miR-18a促进肝癌细胞的侵袭和迁移能力,其机制可能与Dicer1蛋白表达抑制有关。

Objective To investigate the effect of miR-18a on the invasion and migration of hepatoma cells and its mechanism. Methods HepG2.2.15 and HepG2 cells were cultured, passaged, cryopreserved and resuscitated by liposome mediated transfection and were transfected with miR-18a inhibitor and miR-18a inhibitor negative control respectively. Transwell invasion and migration assays were used to determine the invasion and migration ability of the cells after 24 h. HepG2 and HepG2.2.15 cells were divided into groups and transfected with miR-18a inhibitor, miR-18a inhibitor negative control, co-transfected with miR-18a inhibitor and Dicer siRNA, and co-transfected with miR-18a inhibitor and Dicer siRNA negative control for 48 h respectively. The differences of Dicer1 protein expression between different groups were compared by transwell invasion and migration assays. Results After transfection with miR-18a inhibitor, the invasion and migration ability of HepG2 and HepG2.2.15 cells were decreased significantly (P<0.05). The expression of Dicer1 protein in cells transfected with miR-18a inhibitor was significantly higher than that in cells transfected with miR-18a inhibitor negative control (P<0.05). The expression of Dicer1 protein in Dicer siRNA + miR-18a inhibitor group was lower than that in Dicer siRNA negative control + miR-18a inhibitor group (P<0.05), while the invasion and migration ability of cells were increased significantly (P<0.05). Conclusion miR-18a can promote the invasion and migration of hepatoma cells, and its mechanism may be related to the inhibition of Dicer1 protein expression.

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