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miR-125b通过靶向致癌基因LIN28B调控肝细胞癌细胞的增殖和侵袭行为
作者:李建华  韩玲  杨志良 
单位:新乡市中心医院普外科  河南 新乡 453000 
关键词: 肝细胞/病理学 癌基因/遗传学 微RNAs 锌指 基因表达 肿瘤侵润 细胞增殖 肿瘤细胞 培养的 聚合酶链反应 
DOI:R735.7;R730.23
出版年,卷(期):页码:2018,33(2):128-132
摘要:

目的 探讨miR-125b通过靶向致癌基因LIN28B调控肝细胞癌的增殖和侵袭行为及其机制。方法 qPCR检测肝癌和癌旁正常肝组织中miR-125b和LIN28B的表达情况。双荧光素酶实验分析293U细胞中miR-125b和LIN28B之间的相互关联。MTT增殖实验检测miR-125b对肝癌HepG2细胞增殖能力的变化。Transwell侵袭实验检测miR-125b对HepG2细胞侵袭能力的变化。结果 与癌旁正常肝组织比较,肝癌组织中miR-125b和LIN28B表达增高(均P<0.05)。双荧光素酶实验证明miR-125b能与LIN28B的3'UTR特异性结合。抑制miR-125b可以抑制肝癌细胞增殖和侵袭能力(均P<0.05)。结论 miR-125b在肝癌发生和发展过程中发挥作用,可能是通过调控LIN28B的表达影响肝癌细胞的增殖和侵袭能力。

Objective To investigate the effect and mechanism of miR-125b on the proliferation and invasion of hepatocellular carcinoma cells by targeting the oncogene LIN28B. Methods The expression of miR-125b and LIN28B in hepatocellular carcinoma and adja365bet娱乐官网网址cent normal liver tissues was detected by qPCR. Double luciferase was used to analyze the interrelation between miR-125b and LIN28B in 293U cells. MTT proliferation assay was used to examine the effect of miR-125b on the proliferation of hepatoma HepG2 cells. Transwell invasion assay was used to analyze the impact of miR-125b on the invasive ability of hepatoma cells. Results Compared with adjacent normal liver tissues, the expression of miR-125b and LIN28B was significantly increased in hepatocellular carcinoma tissue (both P<0.05). Double luciferase experiments demonstrated that miR-125b specifically binds to the 3'UTR of LIN28B. Moreover, the proliferation and invasion of hepatocarcinoma cells can be inhibited by blocking miR-125b (both P<0.05). Conclusion miR-125b plays an important role in the progression of hepatocellular carcinoma, which may influence the proliferation and invasion of hepatocarcinoma cells by regulating the expression of LIN28B.

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