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Vimentin、TP53和podoplanin在卵巢癌中的表达水平及预后价值分析
作者:寇建芳  苑中甫 
单位:郑州市第二人民医院妇科  河南 郑州 450000 
关键词:卵巢肿瘤/病理学 波形蛋白/代谢 肿瘤蛋白质类/代谢 膜糖蛋白类/代谢 免疫组织化学 预后 
DOI:R737.31;R730.23
出版年,卷(期):页码:2018,33(3):255-262
摘要:

目的 探究波形蛋白(vimentin)、肿瘤蛋白p53(tumor protein p53,TP53)和平足蛋白(podoplanin)在卵巢癌中的表达及预后价值。方法 收集116例卵巢癌组织标本作为卵巢癌组,另选取90例正常卵巢组织标本作为对照组,RT-PCR法和免疫组织化学法测定卵巢组织中vimentin、TP53和podoplanin表达,分析vimentin、TP53和podoplanin蛋白表达与临床病理特征的关系。采用Kaplan-Meier法对3年生存情况进行分析,Log-rank法检测组间生存差异;COX风险回归对影响卵巢癌患者的无生存进展期(progression-free survival,PFS)和总体生存期(overall survival,OS)进行分析。结果 卵巢癌组vimentin、TP53和podoplanin mRNA表达水平均高于对照组(均P<0.05)。免疫组织化学检测结果显示,vimentin、TP53和podoplanin蛋白阳性表达以及平均吸光度均高于对照组(均P<0.05)。Vimentin表达与临床分期、淋巴结转移、组织分化程度相关(U=37.286、19.041、35.478,均P<0.05),TP53表达与组织分化程度相关(U=25.481,P<0.05),podoplanin表达与年龄相关(U=24.471,P<0.05)。Vimentin、TP53和podoplanin蛋白阳性表达者3年PFS和OS均低于阴性表达者(均P<0.05)。Cox风险回归分析结果显示,年龄和vimentin表达是影响卵巢癌患者PFS和OS的独立预后因素。结论 Vimentin、TP53和podoplanin在卵巢癌组织中高表达,其中vimentin可作为卵巢癌患者独立预后指标。

Objective To explore the expression and prognostic value of vimentin, tumor protein p53 (TP53) and podoplanin in ovarian cancer.Methods One-hundred-and-sixteen specimens of ovarian cancer were selected as the ovarian cancer group, and 90 specimens of normal ovarian tissues were selected as the control group. The expression levels of vimentin, TP53 and podoplanin in ovarian tissues were analyzed by RT-PCR and immunohistochemical methods. The relationship between protein expression and clinicopathological parameters was analyzed. Kaplan-Meier method was used to analyze the 3-years survival rates. Log-rank method was used to detect the survival difference between the groups. Cox risk regression analysis was used to study the progression-free survival (PFS) and overall survival (OS) of ovarian cancer patients.Results The levels of vimentin, TP53 and podoplanin in the ovarian cancer group were all significantly higher than those in the control group (all P<0.05). Besides, immunohistochemistry results suggested that the positive expression and mean absorbance value of vimentin, TP53 and podoplanin were also obviously higher than those in the control group (all P<0.05). Moreover, the expression of vimentin was correlated with clinical stage, lymph node metastasis and tissue differentiation (U=37.286,19.041,35.478,all P<0.05); the expression of TP53 was correlated with the degree of differentiation (U=25.481,P<0.05), and the expression of podoplanin was correlated with age (U=24.471,P<0.05). Finally, those patients who had positive expression of vimentin, TP53 and podoplanin showed significantly lower 3-year PFS and OS than those with negative protein expression (both P<0.05). Cox regression analysis suggested that age and vimentin expression were independent prognostic factors of PFS and OS in patients with ovarian cancer.Conclusion Vimentin, TP53 and podoplanin are highly expressed in ovarian cancer tissues, and vimentin can be used as an independent prognostic indicator for patients with ovarian cancer.

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